standard microbiological test

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Question:

Ames test is a standard microbiological test required of candidate drugs in development.

Which statement applies to the Ames test?

The Ames test is used in pre-clinical testing of candidate drugs to demonstrate the ability of antibodies to bind to target antigens.

The Ames test looks at the restoration of the ability to biosynthesize histidine by what was his-Salmonella mutants.

In the Ames test, if exposed to a mutagen, many more colonies will be able to grow in plates with excess histidine.

The Ames test is required by FDA for investigational drugs. However, its not required by the EPA for new chemicals that will be marketed.

In the Ames test, liver extract is added because liver enzymes serve as nutrition source of Salmonella.

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